Revolutionising Gene Expression Diagnostics: Introducing DIRECT LS-TA
Our company stands at the forefront of innovation in gene expression diagnostics, transforming how we understand host responses and disease. Our debut product, DIRECT LS-TA (Direct Leukocyte Single cell-type Transcript Abundance), embodies our innovative approach to analysing complex cell-mixture biological samples without laborious cell sorting.
Get Product Information
The Problem with Traditional Blood Analysis
Bulk Expression Limitations
Traditional whole blood gene expression analysis combines signals from multiple cell types, masking critical single cell-type responses. This creates diagnostic ambiguity when you need precise cellular insights.
  • Mixed signals from 5+ blood cell types (e.g. monocyte, granulocyte, various lymphocytes)
  • Loss of cell-specific diagnostic markers
  • Requires expensive cell isolation steps
Game-Changing Solution: DIRECT LS-TA Technology
Direct Blood Analysis
Analyze whole blood samples without costly and time-consuming cell isolation procedures. Get results in hours, not days.
Single Cell-Type Precision
Obtain accurate gene expression data for specific cell populations using our patented ratio-based biomarker approach.
Clinical-Grade Accuracy
Validated against gold standard methods with excellent correlation (R² > 0.9) for clinical decision making.
Clinical Applications
Triage of febrile patients by major causes
Rapidly distinguish between bacterial and viral infections using specific biomarker signatures:
  • Bacterial: Elevated VNN1/PSAP ratios indicating cytokine activation
  • Viral: Increased IFI27/PSAP ratios showing interferon response
  • Normal: Both markers within reference ranges
Antimicrobial stewardship: the test can rule out bacterial infection, no antibiotics is needed for a patient. Evidence based approach to help the global crisis of antimicrobial resistance.
< 3
Hours to Results
Compared to days for traditional methods
> 85%
Accuracy Rate
Validation studies
Advanced PCR Platform Integration
Assay now available on QCPR and dPCR
2nd Generation: Dual Target
VNN1 (FAM) and IFI27 (HEX) detection in single reaction for basic bacterial vs viral differentiation.
3rd Generation: Quad Target
Four genes in one dPCR reaction enabling comprehensive multi-cell-type analysis with enhanced precision.
4th Generation: Complete Panel
Separate monocyte and granulocyte reagent kits with 3 targets plus 1 reference gene per reaction.
Advantages

Award Recognition: Gold Award winner at the 48th International Exhibition of Inventions of Geneva, validating our innovative approach to single cell diagnostics.
Validated Clinical Results
Real Patient Case Studies
Patient 1: Viral/SLE Detection
VNN1/PSAP: 0.1% (normal)
IFI27/PSAP: 46% (40x elevated)
Diagnosis: Strong interferon activation consistent with viral infection or SLE
Patient 2: Bacterial Infection
VNN1/PSAP: 4.3% (4x elevated)
IFI27/PSAP: 0.8% (normal)
Diagnosis: Proinflammatory cytokine activation indicating bacterial infection
Patient 3: No Active Infection
VNN1/PSAP: 0.9% (normal)
IFI27/PSAP: 0.8% (normal)
Diagnosis: Neither pathway activated, ruled out infectious etiology
Products
Reagent kits for DIRECT LS-TA Assay. FOR RESEARCH USE ONLY.
Works with common brands of both qPCR and Digital PCR machines. Taqman and primers only kits. *Master mix NOT provided.
Partner With Innovation
Ready to Transform Your Diagnostics?
Join leading pharmaceutical companies and research institutions already implementing DIRECT LS-TA technology. Our expert team provides comprehensive support from implementation to clinical validation.
  • Reagent kits and protocols
  • AI and Bioinformatics Services
Contact:
Nelson L.S. TANG, MBChB, MD, FRCPA
Contact [email protected] to buy product
Learn More

directlsta.org

Revolutionising Gene Expression Diagnostics: Introducing DIRECT LS-TA

Our company stands at the forefront of innovation in gene expression diagnostics, transforming how we understand host responses and disease. Our debut product, DIRECT LS-TA (Direct Leukocyte Single cell-type Transcript Abundance), embodies our innovative approach to analysing complex cell-mixture bi